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Image Search Results
Journal: PLoS ONE
Article Title: Quantitative Resistance of Potato to Pectobacterium atrosepticum and Phytophthora infestans: Integrating PAMP-Triggered Response and Pathogen Growth
doi: 10.1371/journal.pone.0023331
Figure Lengend Snippet: For P. atrosepticum (A), rot weight was measured 5 days after inoculation of half tubers; for P. infestans (B), disease severity was expressed as the reflective density deduced with the image analysis software ImageJ from levels of grey recorded on tuber slices scanned 15 days after inoculation with the pathogen. Experiments were performed on tubers from five potato cultivars (ac = Ackersegen, bf = BF15, bi = Bintje, ke = Kerpondy, sa = Saturna), produced in 2008 and 2009. Tubers produced in 2008 were used after 8 months of storage (tuber batch A), tubers produced in 2009 were used after 2 months (tuber batch B) and after 10 months (tuber batch C) of storage. Data for batch C are means from two replicate experiments (vs. one for tuber batches A and B); for each cultivar and treatment, five tubers were used in each experiment. Bars correspond to means ± standard error. Different letters above bars indicate significant differences between means of cultivars within a batch (Tukey's test, p<0.05).
Article Snippet: After incubation for 15 days at 17°C in a dark and water-saturated ambience, tubers were sliced transversely and the reflective density was measured individually on four sections using a desk scanner (Epson Perfection 1260) calibrated to density with a
Techniques: Software, Produced
Journal: PLoS ONE
Article Title: Quantitative Resistance of Potato to Pectobacterium atrosepticum and Phytophthora infestans: Integrating PAMP-Triggered Response and Pathogen Growth
doi: 10.1371/journal.pone.0023331
Figure Lengend Snippet: Tuber slices of cultivar Bintje were surface-treated with 100 µl of a concentrated culture filtrate (CCF) of Phytophthora infestans at a concentration of 400 µg.ml −1 or with water as the control. Slices were incubated during 7.5 h at 20°C and subsequently assayed for Phenylalanine ammonia-lyase (PAL) activity (A) or inoculated with pathogens. For Pectobacterium atrosepticum (B), weight of soft rot developed during 5 days at 20°C was measured. For P. infestans (C), disease developed during 5 days at 17°C and is expressed as reflective density. PAL activity was determined in four aliquot samples prepared form pooled tissue of 5 tuber slices and disease severity was determined in 10 tuber slices. Bars correspond to means ± standard error of data from two replicate experiments. Different letters above bars indicate significant differences between means of treatments (Tukey's test, p<0.05).
Article Snippet: After incubation for 15 days at 17°C in a dark and water-saturated ambience, tubers were sliced transversely and the reflective density was measured individually on four sections using a desk scanner (Epson Perfection 1260) calibrated to density with a
Techniques: Concentration Assay, Control, Incubation, Activity Assay